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KMID : 0903519920350050420
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
1992 Volume.35 No. 5 p.420 ~ p.421
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Molecular genetic analysis of the genes for salinomycin biosynthesis in Streptomyces albus
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Kwon, Hyung Jin
Kato, Fumito/Hutchinson, C R/Suh, Joo Won
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Abstract
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Streptomyces albus wild type produced salinomycin and the strain 14∼33 chromosomal DNA was used for the construction of genomic library using pKC 505 cosmid vector. The genomic library was screened using act 1 (gene for actinorhodin synthase-condensing enzyme) probe and p22∼81 was isolated. TLC, HPLC, and MS analyses revealed that S. lividans transformed with the p22∼81 produced salinomycin. The p22∼81 contained about 24 kb insert and a region of 4.4 kb fragment of the insert hybridized with actI and ACP (acyl-carrier protein) probes was sequenced. Five ORFs found in the same direction were homologus to tcmI, tcmK, tcmL, tcmM and actIII, respectively (tcm; tetracenomycin, act; actinorhodin), tcmI is a cyclase in tetracenomycin synthase gene clusters and tcmK & tcmL are gene for heterodimeric beta-acyl synthase. tcmM codes ACP and actIII is a gene for ketoreductase.
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